HotStar All in One

Cat. No. HQ0100
Size: 100 reactions
Store at 4 or -20°C
Description
HotStar All in One provides qualified reagents for the amplification of nucleic acid templates by polymerase chain reaction (PCR). The mixture contains anti-Taq DNA polymerase antibody, Mg++, dNTPs, and recombinant Taq DNA polymerase at concentrations sufficient to allow amplification during PCR. HotStar All in One is supplied at 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 100 amplification reactions of 50 μl each are provided. Due to specific binding of the antibody, HotStar All in One is present in an inactive form and is reactivated after a denaturation step in PCR cycling at 94°C. HotStar All in One may be stored at either -20°C or 4°C. No detectable reduction of PCR performance or enzyme activity is observed after storage of HotStar All in One for 12 months at 4°C. Repeated freeze-thaw cycles do not reduce performance or activity.

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